Protein Expression Scientist

Our large client in the pharmaceutical industry is looking for a Protein Expression Scientist to work in a fast-paced R&D environment applying rigorous scientific principles towards groundbreaking biological therapeutics. A successful candidate will directly employ molecular biology, cell culture and biochemistry techniques to discover our next medicines. In this capacity, the Scientist will primarily express monoclonal antibodies, multispecifics, and other biomolecules to enable robust in vitro characterization and in vivo studies. The candidate will use mammalian cell culture, transient and stable expression systems, and analytical techniques to produce and evaluate expression supernatants. The candidate will also use various molecular biology and biochemical analytical methods to characterize transfection-ready plasmid DNA and purified proteins. An outstanding candidate will be driven by a desire to collaborate, expertise of scientific principles, and working adeptly within data informed timelines.

Essential Functions:
· Draw upon knowledge and hands-on experience with cell culture and other analytical methods to express proteins up to gram scale in CHO, HEK293, and hybridomas.
· Perform characterization assays such as BLI, A280/A260 measurement, SDS-PAGE, HPLC, endotoxin testing, MALS, cIEF, CE-SDS, etc. to evaluate the quality of DNA, expression supernatants and proteins
· Design, execution, and interpretation of experiments with manager’s approval.
· Maintenance of accurate laboratory notebooks in a timely fashion and prepare technical reports, summaries, protocols, etc.
· Support efforts to source protein production with CROs and external partners.
· Collaboration and communication with other scientists in a multifaceted and dynamic research environment.

We are a company committed to creating diverse and inclusive environments where people can bring their full, authentic selves to work every day. We are an equal opportunity/affirmative action employer that believes everyone matters. Qualified candidates will receive consideration for employment regardless of their race, color, ethnicity, religion, sex (including pregnancy), sexual orientation, gender identity and expression, marital status, national origin, ancestry, genetic factors, age, disability, protected veteran status, military or uniformed service member status, or any other status or characteristic protected by applicable laws, regulations, and ordinances. If you need assistance and/or a reasonable accommodation due to a disability during the application or recruiting process, please send a request to HR@insightglobal.com.To learn more about how we collect, keep, and process your private information, please review Insight Global's Workforce Privacy Policy: https://insightglobal.com/workforce-privacy-policy/.

· A minimum of a bachelor’s degree in Biochemistry or related field with 5+ years of experience, or an MS with 3+ years of experience, or a PhD with 1+ years of experience
· Prior hands-on experience with aseptic mammalian cell culture in shake flasks
· Expertise with chemical based transient transfection and electroporation of CHO and HEK293
· Scale up of biologics from hybridomas and cell lines using chemically defined media
· Experience using analytical techniques BLI, A280/A260, DNA and protein electrophoresis
· An understanding of therapeutic proteins and the drug discovery process
· Experience in maintaining a laboratory notebook and databases

· Experience with roller bottles and bioreactors such as DASGIP and Amber250
· Experience with analytical techniques HPLC, MALS, cIEF, CE-SDS, and ELISA
· Experience with bispecific or multi-specific antibodies, novel protein therapeutics, and antibody mechanisms of action
· Protein purification experience using AKTA FPLC

Benefit packages for this role will start on the 1st day of employment and include medical, dental, and vision insurance, as well as HSA, FSA, and DCFSA account options, and 401k retirement account access with employer matching. Employees in this role are also entitled to paid sick leave and/or other paid time off as provided by applicable law.